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. 2015 May 19;6:161. doi: 10.3389/fphys.2015.00161

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Copyright © 2015 McClung, Reinardy, Mueller, McCord, Kontos, Brown, Hussain, Schmidt, Ryan and Green.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Angiopoietin-1 enhances myotube formation. (A–D) Myoblasts were grown to confluence and allowed to differentiate for 48- or 96-h. QRT-PCR was used to determine the mRNA expression levels of Ang-1, Ang-2, Tie1, and Tie2. (E–G) Myoblasts were grown to confluence and allowed to differentiate for 24 h with exogenous administration of recombinant hepatocyte growth factor (HGF; 500 ng/mL), Ang-1 (500 ng/mL), or Ang-2 (500 ng/mL). QRT-PCR was used to determine the mRNA expression of the cell cycle regulator p21 (E) and the myogenic regulatory factors MyoD (F) and Myogenin (G). (H) Myoblasts differentiated for 24-h with exogenous HGF, Ang-1, or Ang-2 were analyzed for myosin heavy chain (MyHC) protein. ^*P < 0.05 vs. Myoblast. All values are means ± SE.