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. 2015 May 19;6:161. doi: 10.3389/fphys.2015.00161

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Copyright © 2015 McClung, Reinardy, Mueller, McCord, Kontos, Brown, Hussain, Schmidt, Ryan and Green.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of Ang-1 on pluripotent cell myotube formation. (A,B) Mixed primary isolates including peri-endothelial progenitors and MPCs were immunofluorescently labeled (A) or western blotted (B) to verify NG2 and/or Pax7 expression to demonstrate the relative expression of NG2 and smooth muscle actin (SMA) compared to that in immortalized C2C12 myoblasts. (C) Ang-1 effects on mixed cell isolate differentiation into myotubes (Fusion Index, %). (D) C3H-10T1/2 pluripotent cells and C2C12 myoblasts were plated at a 75:25 ratio. Cells were allowed to adhere and then treated with Ang-1 and analyzed for myoblast fusion rates after 120 h of low serum culture. (E,F) Effects of inhibiting bio-available Ang-1 in MC CM on myotube formation in C3H-10T1/2:C2C12 co-cultures. Co-cultures were infected with either control AdGFP, or AdsTie2 for 24 h and analyzed for myoblast fusion after a total of 120 h of reduced serum culture with Ang-1 or MC CM supplementation. ^*P < 0.05 vs. Control or virus-matched Control. ^†P < 0.05 vs. AdGFP MC CM. β P = 0.08 vs. AdExTEK Control. All values are means ± SE.