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. 2015 May 19;6:161. doi: 10.3389/fphys.2015.00161

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Copyright © 2015 McClung, Reinardy, Mueller, McCord, Kontos, Brown, Hussain, Schmidt, Ryan and Green.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Conditioned medium from ischemic muscle cells enhances endothelial cell mesh/loop formation. (A) HUVECs were plated on Matrigel and imaged after 8-h of treatment in medium supplemented with 25% by volume vehicle (HBSS), Ang-1 (500 ng/mL), conditioned medium from experimentally ischemic myotubes (MC CM), or conditioned medium from experimentally ischemic myotubes (MC CM) after Ang-1 depletion with Tie2-Fc. (B) Analysis of mesh/loop formation. For six replicate experiments the mean mesh/tube number was calculated from three fields of view. ^*P < 0.05 vs. HBSS. ^† vs. rhAng1 or MC CM. All values are means ± SE.