Table 2.
Morphological and genetic characterization of the diploid and the tetraploid hybrid aspen lines under optimal water supply (stem height was measured at the beginning of the experiment after 9 month in soil, other parameters were measured at the end of the experiment.
| Line | Fusion partners | Leaf morphology | Stem height [m * 10−2] | Stem biomass [g plant−1] | Total leaf area [m2* 10−2 plant−1] | Leaf mass per area [g m−2* 10] | Ploidy level | nSSR |
|---|---|---|---|---|---|---|---|---|
| 27-01 | – | P3 | 105.1 ± 17.6c | 17.5 ± 8.3b | 30.4 ± 7.7b,c | 1.38 ± 0.15a | 2n | P3 |
| 27-09 | P3 × P9 | P3 | 39.6 ± 17.3a | 1.9 ± 1.2a | 6.5 ± 1.7a | 2.26 ± 1.98c | 4n | P3 |
| 27-10 | P3 × P9 | P3 | 80.3 ± 16.7b | 16.3 ± 10.0b | 23.9 ± 12.7b,c | 1.59 ± 0.23b | 4n | P3 |
| 27-11 | P3 × P9 | P3 | 49.8 ± 11.8a | 4.9 ± 3.5a | 15.4 ± 7.8a, b | 1.53 ± 0.26a,b | 4n | P3 |
| 27-12 | P3 × P7 | P3 | 92.8 ± 18.4b, c | 22.1 ± 7.3b | 34.2 ± 6.0c | 1.69 ± 0.21b | 4n | P3 |
For analyzing ploidy level and nSSR in vitro material was used. Data are means ± SE, n = 10 plants (stem height and stem biomass), n = 6 plants (total leaf area and leaf mass per area), superscripted letters indicate differences between the lines (Tukey HSD test, p < 0.05), P3: P. tremula × P. tremuloides, P7: P.nigra, P9: P. trichocarpa × P. deltoides).