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. 2015 May 19;6:159. doi: 10.3389/fphys.2015.00159

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Copyright © 2015 Alekov.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Proton transport mediated by mutation G212A. (A) Representative alkalinization recorded at different voltages in a cell transfected with mutation G212A ClC-5 using ratiometric BCECF fluorometry. Lines represent linear fits to the data and were used to provide the rates of alkalinization ΔpH/Δt. (B) Averaged rates of alkalinization normalized to the value measured at +140 mV (n = 5). The red line represents the current–voltage relation of G212A ClC-5 depicted in Figure 5A and analogously normalized to the current measured at +140 mV.