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. Author manuscript; available in PMC: 2015 May 19.
Published in final edited form as: J Immunol. 2009 Nov 30;184(1):94–104. doi: 10.4049/jimmunol.0900753

Figure 5.

Figure 5

Fas is required for plate-bound Ab-induced apoptosis. A, CD4+CD25− T cells from C57.BL/6 (B6), Fas-deficient (lpr), or TNF-R–deficient (TNFR−/−) mice were stimulated with plate-bound anti-CD3 and anti-CD28 Abs. After 5 d, the cells were harvested and stained for Annexin V and 7-AAD, followed by analysis using a flow cytometer. B, CD4+CD25+ or CD4+ CD25− T cells from B6 mice were stimulated with plate-bound anti-CD3 Ab or anti-CD3 and anti-CD28 Abs for 3 d. At the end of the culture period, the cells were harvested and analyzed for Fas and FasL expression by flow cytometry. The percentage of cells in each rectangle is shown.