Table 1.
Effect of transplant cell dose and time on the efficiency of liver repopulation in vivo after HTx
| Time of Tx (mo) |
Kinetics of repopulation |
Effect of cell dose on repopulation |
|||||||
| 1 | 2 | 3 | 20 | 3 | 3 | 3 | 3 | 3 | |
| Infused cell number Post Tx | 2×106 | 2×106 | 2×106 | 1×106 | 1×106 | 1×105 | 1×104 | 1×13 | 1×102 |
| Liver repopulation (%) | 14.8±4.2 | 50.7± 13.9 | 70.4?±9.0 | 89.8± 4.7 | 51.0± 4.7 | 28.9± 7.2 | 13.8± 5.0 | 1.1± 1.2 | 0.6± 0.6 |
| Colony number/mm2 | 5.48± 1.10 | 5.1±.1.40 | NA | NA | 2.64±0.16 | 0.91±0.05 | 0.37±0.39 | 0.09±0.07 | 0.01±.01 |
| Diameter of colony (μm) | 149.4± 110.5 | 280.1±219.4 | NA | NA | 425.2± 278.8 | 376.7± 64.8 | 476.5± 54.5 | 354.5± 108.9 | 698.7± 39.2 |
| Number of animals | 6 | 6 | 3 | 4 | 3 | 3 | 4 | 4 | 4 |
| Area examined (mm2) | 365.0 | 402.0 | 66.6 | 2386.5 | 77.6 | 284.1 | 464.8 | 458.0 | 470.5 |
Donor cells were derived from DPPIV+ rats. Recipients were DPPIV-deficient rats treated with retrorsine and two-thirds PH; DPPIV+ cells were visualized by their enzymatic activity after histochemical staining of cryostat sections. The characteristics of repopulation, colony number and colony diameter were analyzed based on DPPIV+ cells. Computer-aided area analysis was used for quantitative detection of repopulation. All of the data presented as mean± SD from three to six individuals in two or three independent experiments. HTx: hepatocellular transplantation; Tx: transplantation; NA: not applicable.