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. 2015 Jun;21(6):1159–1172. doi: 10.1261/rna.045559.114

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© 2015 Liu et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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FIGURE 2. — Characteristics of YB-1–associated short RNAs. (A) Analysis of distinct YB-1–associated short RNAs (shyRNAs) after removal of input RNAs. (B) The RNY3 RNA is matched to YB-1–associated shyRNAs (blue) and low abundance small RNAs (black) that preferentially derive from either the 5′ or 3′ ends of RNY3. The y-axis represents the number of reads while the x-axis shows the genomic location of the reads. The most abundant read is nearly identical to RNY3. (C) Small RNAs preferentially match to the 5′ and 3′ ends of shyRNAs. (D) Correlation between shyRNAs, small RNAs, and mRNAs in PC-3 cells. (E) qPCR validation of different classes of small and shyRNAs.