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. Author manuscript; available in PMC: 2015 May 19.
Published in final edited form as: Methods Mol Biol. 2014;1123:1–26. doi: 10.1007/978-1-62703-968-0_1

Table 2.

Comparison of protein-targeted genome engineering

HE ZFN TALEN
DNA ends at DSB 3′ extension 5′ extension 5′ extension
Sequence availability Limited by existing enzymes Limited by triplet recognition of ZFs Limited by first nucleotide (T)
Context effects Common Common Uncommon
Specificity High Intermediate High
Off-target effects Low Intermediate Low
Enzyme engineering Complex Intermediate Straightforward
Size compactness (modularity) Small (monomeric) Large (monomeric and dimeric) Very large (dimeric)