Table 1.

Ru fraction [mol/mol(protein)] in ruthenated Tf or HSA produced after (1) incubation of NAMI-A, Tf and HSA at 1:1:1 molar ratio for 24 h in buffer; (2) incubation of HSA with 20 molar excess of NAMI-A, followed by purification by FPLC separation from free Ru species, and then mixed with Tf at 1:2 molar ratio

	Model	Molar ratio	Ru/Tf	Ru/HSA
(1)	holo-Tf/HSA/NAMI-A	1:1:1	0.39 ± 0.08	0.65 ± 0.07
	apo-Tf/HSA/NAMI	1:1:1	0.24 ± 0.09	0.56 ± 0.06
(2)	(HSA/NAMI)/holo-Tf	(1:1):2	0.31 ± 0.02	0.67 ± 0.03
	(HSA/NAMI)/apo-Tf	(1:1):2	0.23 ± 0.02	0.71 ± 0.05

Buffer conditions: 50 mM TRIS/HCl, 100 mM NaCl, 25 mM NaHCO₃, pH 7.4, 37 °C