Fig. 3.

Mutations in low-frequency genes can affect the subcellular localization of proteins. HOG and HEK 293 cells were transiently transfected with either the wildtype or mutant construct and stained for GFP (green), DAPI (blue) and Alexa fluor phalloidin (red). A total of twelve wildtype and mutant construct pairs were made. In both cell lines, the mutated NTN4 construct showed a stronger nuclear staining pattern compared to the wildtype construct. For MAGEH1, the mutation resulted in a reduced or even absent nuclear staining. No differences in the subcellular localization between wildtype and mutant constructs were found in HOG and HEK 293 cells expressing GDI1, ZNF238, SASH3, XPO7, ZNF57, GABRE, OR5D14, PGLYRP4, ARSE and DCUN1D2 (not shown)