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. 2015 Apr 14;129(6):895–907. doi: 10.1007/s00401-015-1415-2

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© The Author(s) 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 3 — Immunoblot analysis of the effect of APP on seed-dependent tau aggregation in cultured cells. Immunoblot analysis of lysates from cells transfected with APP, cells transfected with 4R1N tau, cells transfected with both 4R1N tau and APP, cells transfected with APP and treated with 4R1N tau fibrils, cells transfected with 4R1N tau and treated with 4R1N tau fibrils, and cells transfected with both 4R1N tau and APP and treated with 4R1N tau fibrils (a). Immunoblot analysis of lysates from cells transfected with only APP, and cells transfected with both APP and 3R1N tau and treated with 3R1N tau fibrils (b). Immunoblot analysis of lysates from cells transfected with both APP and 4R1N tau and treated with 4R1N tau monomers or 4R1N tau fibrils (c). Cells were extracted successively to obtain Tris–HCl-soluble fraction (TS), Triton X-100 soluble fraction (TX), and Sarkosyl-soluble fraction (Sar), leaving the pellet fraction (ppt). Tau was detected with HT7 (159–163), T46 (395–432), pS396 (p-Ser-396), and AT8 (p-Ser-202 and p-Thr-205). APP was detected with 22C11