Glass slide partially coated with AdheSE® One F containing 2.5 wt% DDA-X-PMOx28-AMA, photo-cured, washed with water for 2 d, dried, sprayed with S. mutans cells in 0.1 M PBS buffer (pH 7.0, 105 cells/mL), incubated under growth ager (1.5 wt% agar) at 37°C overnight, and stained with TTC.