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. 2015 May 10;13:39. doi: 10.1186/s12958-015-0036-x

Figure 1.

Figure 1

Synchronization protocol. Cows were pre-synchronized via two injections of prostaglandin F2α (PGF) administered 14 days apart, starting on protocol Day −34 (D–34). On D–10, the cows received a progesterone-releasing device (P4 device) (Sincrogest; Ourofino Saúde Animal, Cravinhos, SP, Brazil) and an injection of 2 mg of estradiol benzoate (EB; Sincrodiol; Ourofino Saúde Animal). Also, on D–10, the cows were prearranged between large follicle-large corpus luteum group (LF-LCL) or small follicle-small corpus luteum group (SF-SCL) and only cows of LF-LCL received an injection of PGF (0.5 mg of sodium cloprostenol; Sincrocio; Ourofino Saúde Animal). Between D–1.75 and D–2.5 the P4 device was removed from cows of LF-LCL. On the cows of SF-SCL the P4 device was removed between D–1.25 and D–1.5. All the animals received an injection of PGF in the same time of P4 device withdrawal and a second PGF injection 6 h later. On D0, in both groups, the ovulation was induced with an injection of gonadotropin-releasing hormone (GnRH; 0.01 mg of buserelin acetate; Sincroforte; Ourofino Saúde Animal). Blood sampling was conducted on D0, D2, D6 and D7. The cows were slaughtered on D7, which was the end point for endometrial tissue collection.