Table 1.
Experimental design
| Procedure | Daytime | |
|---|---|---|
| SF–SCL | LF–LCL | |
| First PGF of pre-synchronization | D–34 | D–34 |
| Second PGF of pre-synchronization | D–20 | D–20 |
| P4 device insertion + EB injection | D–10 | D–10 |
| PGF* | NO** | D–10 |
| P4 device withdrawal + PGF*** | D–1.25 to D–1.5 | D–1.75 to D–2.5 |
| GnRH injection | D0 | D0 |
| Blood sampling | D0, D2, D6 and D7 | D0, D2, D6 and D7 |
| Slaughter | D7 | D7 |
Abbreviations: PGF prostaglandin F2α (sodium cloprostenol; Sincrocio®, Ourofino Saúde Animal, Cravinhos, Brazil), P4 device progesterone-releasing device (Sincrogest; Ourofino Saúde Animal, Cravinhos, SP, Brazil), EB Estradiol benzoate (Sincrodiol®, Ourofino Saúde Animal), GnRH gonadotropin-releasing hormone (Sincroforte®, Ourofino Saúde Animal). *PGF given on the beginning of protocol; **Cows of SF-SCL did not receive PGF injection on D–10; ***PGF given on the end of protocol.