Table 2.
Characteristics of the primers used for qPCR
| Target gene | GenBank ID | Primer sequence (5’-3’) | Amplicon (bp) | Reference | |
|---|---|---|---|---|---|
| SOD1 | NM_174615.2 | F | GTTGGAGACCTGGGCAATGT | 151 | Primer Express* |
| R | TCCACCCTCGCCCAAGTCAT | ||||
| SOD2 | NM_201527.2 | F | CCCATGAAGCCTTTCTAATCCTG | 307 | [47] |
| R | TTCAGAGGCGCTACTATTTCCTTC | ||||
| SOD3 | NM_001082610.1 | F | GAGAGCGAGTGTAAAGCCGT | 190 | PrimerQuest** |
| R | CCTGGAAGAGGCACACAGAG | ||||
| CAT | NM_001035386.2 | F | CGCGCAGAAACCTGATGTC | 150 | Primer Express* |
| R | GGAATTCTCTCCCGGTCAAAG | ||||
| GPX4 | NM_174770.3 | F | TCACCAAGTTCCTCATTGACAAGA | 150 | Primer Express* |
| R | TTCTCGGAACACAGGCAACA | ||||
| PPIA | NM_178320.2 | F | GCCATGGAGCGCTTTGG | 70 | [48] |
| R | CCACAGTCAGCAATGGTGATCT | ||||
| ACTB | NM_173979.3 | F | GGATGAGGCTCAGAGCAAGAGA | 78 | [48] |
| R | TCGTCCCAGTTGGTGACGAT | ||||
| GAPDH | NM_001034034.2 | F | GCCATCAATGACCCCTTCAT | 71 | [48] |
| R | TGCCGTGGGTGGAATCA | ||||
Abbreviations: SOD1 Superoxide dismutase 1, SOD2 Superoxide dismutase 2, SOD3 Superoxide dismutase 3, CAT Catalase, GPX4 Glutathione peroxidase, PPIA peptidylprolyl isomerase A (cyclophilin A), ACTB actin, beta, GAPDH glyceraldehyde-3-phosphate dehydrogenase. ID: GenBank Identification, *Primer sequences obtained using Primer Express software, version 3.0 (Life technologies, Carlsbad, CA, USA), **Primer sequences obtained using PrimerQuestQM software (IDT Technologies, Coralville, IA, USA).