Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 May 1;26(9):1629–1639. doi: 10.1091/mbc.E14-07-1197

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2015 Wu et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

“ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

PMC Copyright notice

FIGURE 1: — Identification of Homer3 as a neutrophil protein that binds Gαi2. (A) Network analysis of the proteins captured by one or more Gαi2 baits after affinity chromatography in pig leukocyte lysates. The baits are shown in gray, and the proteins are shown in blue. For each protein–bait combination, the numbers at the arrows show the counts, and the width of the lines represents the Z_P score. (B) Affinity chromatography with GST-Gαi2 or GST alone for neutrophil lysate containing FLAG-Homer3. Eluted GST-tagged bait plus associated proteins and final wash fraction were subjected to SDS–PAGE and analyzed by immunoblot with anti-FLAG antibody. (C) Affinity chromatography–based test for direct interaction between purified, bacterially expressed Homer3 (prey) and GST-Gαi2 or GST alone (baits). Homer3 directly binds to both GST-Gαi2-GDP and GST-Gαi2-Gpp(NH)p with similar affinity (n = 5; not significantly different). “Beads” refer to baits without Homer3 (prey). Samples were analyzed with SDS–PAGE and stained with CBB. Arrows indicate GST-Gαi2 (66 kDa), Homer3 (47 kDa), and GST (26 kDa).