FIGURE 4:

Homer3 depletion does not affect levels of Pak phosphorylation (a readout of Rac activation) or Akt phosphorylation (a readout of PIP₃ generation). (A, C) Time course of Akt (A) or Pak (C) phosphorylation, measured by Western blot, after uniform 10 nM fMLP stimulation. Total Akt (A) or total Pak (C) was used as loading control. (B, D) Quantification of phosphorylated Akt (B) or Pak (D) for three independent runs of the experiment shown in A and C , respectively. Phosphorylated Akt (B) or Pak (D) was normalized to total Akt (B) or Pak (D).