FIGURE 5:

Wash functions through the Arp2/3 complex, but not the WASH regulatory complex, for tail hemocyte migration. (A–C′) Time-lapse series of ventral surface projections of migrating hemocytes expressing GFP in SWIP^RNAi (A), Strumpellin^RNAi (B), or CCDC53^RNAi (C) embryos. Hemocytes that began their developmental migrations in the tail were tracked for 180 min (A′, B′, C′). (D, E) Quantification of the number of transmigrated tail hemocytes (D) and number of these tail hemocytes that migrate anteriorly (E) in control (Pxn-Gal4), SWIP^RNAi, Strumpellin^RNAi, CCDC53^RNAi, and Arp3^RNAi embryos (n ≥ 20). (F, F′) Time-lapse series of ventral surface projections of migrating hemocytes expressing GFP in Arp3^RNAi embryos. Hemocytes that began their developmental migrations in the tail were tracked for 180 min (F′). All results are given as means ± SEM; p values are indicated. Scale bars, 40 μm (A–C′, F, F′).