FIGURE 3:

MS4A4 colocalizes with KIT and EEA1 in LAD-2 cells. (A) Confocal micrographs of LAD-2 cells transfected with MS4A4:EGFP chimeric protein (green) immunostained for KIT (APC) (red) in the absence (top) or after stimulation with SCF (bottom). (B) Manders coefficient of colocalization for MS4A4 and KIT. (C) Confocal micrographs of LAD-2 cells transfected with MS4A4:EGFP (left) or cells immunostained with anti-MS4A4 followed by AF488-labeled anti-mouse secondary (right). (D) Immunofluorescence confocal micrographs of LAD-2 cells immunostained for mouse anti-MS4A4 (secondary, AF488) and rabbit anti-EEA1 (secondary, AF594) in the absence of SCF (top) or with SCF stimulation for 10 min (bottom). Scale bars, 5 μm. (E) Manders coefficient of colocalization for MS4A4 and EEA1. For B and E, bars are the mean + SEM from the volume of 16 stacks of images from two separate experiments). *p < 0.05, ANOVA with Sidak's posttest.