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. 2015 May 1;26(9):1711–1727. doi: 10.1091/mbc.E14-07-1221

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© 2015 Cruse et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 6: — Silencing MS4A4 alters KIT colocalization with caveolin-1 but not clathrin. (A) Immunofluorescence confocal microscopy of scramble shRNA–treated (left) and shMS4A4- treated (right) LAD-2 cells stained with rabbit anti-clathrin HC (secondary, AF488; green) and mouse anti-KIT (APC, red) left unstimulated or stimulated with SCF for 2 min. Scale bars, 5 μm. (B) Manders coefficient of colocalization of KIT and clathrin HC at times indicated (minutes) after SCF stimulation. (C) Immunofluorescence confocal microscopy of scramble shRNA–treated (left) and shMS4A4-treated (right) LAD-2 cells stained with rabbit anti–caveolin-1 (secondary, AF488; green) and mouse anti-KIT (APC, red) left unstimulated or stimulated with SCF for 2 min. Scale bars, 5 μm. (D) Manders coefficient of colocalization of KIT and caveolin-1 with SCF stimulation time course. For B and D, bars are mean + SEM from the volume of 16 stacks of images from two separate experiments. **p < 0.01, ***p < 0.001, two-way ANOVA.