FIGURE 8:

Silencing MS4A4 promotes mast cell proliferation and migration. (A) LAD-2 human mast cells treated with scramble shRNA or shMS4A4 were cultured under normal conditions and counted at the indicated time points using flow cytometry. Cell counts are presented as viable cells relative to starting number. (B) CD34⁺ peripheral blood–derived mast cell migration was assessed using a Transwell system. Bars are the mean + SEM from three experiments. *p < 0.05, **p < 0.01. (C) Schematic summary of proposed function for MS4A4. MS4A4 directs recruitment of KIT to caveolae, altering trafficking, signaling, and function of KIT.