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. 2015 May 1;26(9):1764–1781. doi: 10.1091/mbc.E14-10-1475

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© 2015 Ghosh, de Campos, et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 5: — Localization of GFP-tagged WT and the indicated single (A) and multiple (B) K → A substituted nodulin chimeras in WT yeast. Corresponding DIC and GFP confocal image panels. Scale bars, 2 μm. For all mutants, 100–235 cells were imaged and scored. All cells expressing the single K₁A and K₆A mutant reporters displayed exclusive localization of reporter to the cytoplasm, whereas all cells expressing the K₅A reporter showed both PM and cytoplasmic localization for the reporter. Otherwise, >99% of the cells expressing single-mutant K → A derivatives showed exclusively PM localization for the reporter.