Table 2.

Acyl-chain composition of PE species with changed levels in LPCAT3-deficient mice

DOI: http://dx.doi.org/10.7554/eLife.06328.014

PE subclass	Acyl-chain composition	LPCAT3+/+ peak area		LPCAT3−/− peak area		KO/WT (%)	p value
		Mean	SEM	Mean	SEM
38:4	16:0–22:4	373,458	20,239	1,073,902	50,565	288	<0.0001
	18:0–20:4	8,394,810	381,499	4,349,617	102,271	52	<0.0001
	18:1–20:3	1,503,041	38,884	689,131	14,846	46	0.0002
40:4	18:0–22:4	1,831,032	80,319	3,803,421	98,424	208	<0.0001
	18:1–22:3	124,638	4846	236,224	23,357	190	0.0016
	20:0–20:4	187,358	8687	45,797	4286	24	<0.0001
	20:1–20:3	35,837	3019	11,721	2388	33	0.0002
40:5	18:0–22:5	523,416	4657	2,056,845	111,780	393	<0.0001
	18:1–22:4	731,947	43,088	1,934,984	35,746	264	<0.0001
	20:1–20:4	224,759	3572	64,708	3066	29	<0.0001

SRM analyses for discrimination of fatty acids were performed for the indicated PE species (n = 5). Anions of the fatty acids indicated at the second position (e.g., 22:4 for 16:0–22:4 PE) were used for selection at Q3. Sample concentration was adjusted based on tissue weight.