Figure 2.

Protein sequence alignment and phylogenetic analysis of selected serotonin receptors from insects and vertebrates. (A) ClustalW alignment of selected serotonin type 2b receptors, with regions of predicted transmembrane domains denoted by red outlined boxes. (B) Phylogenetic analysis using the neighbor-joining method of the deduced Rhopr5HTR2b receptor protein sequence based on the cloned cDNA. Branch lengths on the tree are representative of the number of average number of amino acid substitutions per site. Percent bootstrap support for the clustering of the related sequences is indicated by the numbers adjacent to the nodes. Each protein sequence included in the analysis is identified by the GenBank accession number and the species name. The R. prolixus serotonin receptor identified in the current study (shown in red font) clusters within the clade including insect type 2b serotonin receptors.