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. 2015 May 19;9:175. doi: 10.3389/fnins.2015.00175

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Copyright © 2015 Paluzzi, Bhatt, Wang, Zandawala, Lange and Orchard.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Heterologous functional assay of the R. prolixus serotonin type 2b receptor (Rhopr5HTR2b) in CHOK1-aeq cells. (A) Dose-response curve demonstrating activity of serotonin and an agonist, alpha-methyl 5-HT, on the expressed Rhopr5HTR2b receptor. (B) Several serotonin receptor antagonists blocked the receptor-induced luminescent response, while gramine had no significant effect. (C) All other insect biogenic amines are inactive on the expressed Rhopr5HTR2b receptor.