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. 2015 May 15;26(10):1811–1828. doi: 10.1091/mbc.E14-07-1238

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© 2015 Takada et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 2: — EGFP-Mnk restores DNA damage responses in mnk mutant embryos during the syncytial blastoderm stage. All frames are from the rhodamine channel. (A) An mnk mutant embryo injected with rhodamine-tubulin. Normal spindles formed (inset), and a timely exit from mitosis occurred. Selected frames from Supplemental Movie S2. (B) An embryo wild type for mnk carrying a P[mTb-GAL4VP16] transgene was injected with bleomycin and rhodamine-tubulin. Anastral spindles formed (inset), and mitotic delay (or arrest) occurred. Arrows indicate dropping nuclei from the cortex. Selected frames from Supplemental Movie S3. (C) An mnk mutant embryo carrying a P[mTb-GAL4VP16] transgene was injected with bleomycin and rhodamine-tubulin. Normal spindles formed (inset), and a timely exit from mitosis occurred. Selected frames from Supplemental Movie S4. (D) An mnk mutant embryo expressing EGFP-Mnk was injected with bleomycin and rhodamine-tubulin. Anastral spindles formed (inset), and mitotic delay/arrest occurred. Selected frames from Supplemental Movie S5. Arrows indicate dropping nuclei from the cortex. (E) An mnk-null embryo expressing EGFP-D303A was injected with bleomycin and rhodamine-tubulin. Normal spindles formed (inset), and a timely exit from mitosis occurred. Selected frames from Supplemental Movie S6. Elapsed time is shown in minutes:seconds. Scale bar, 10 μm.