Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 May 15;26(10):1811–1828. doi: 10.1091/mbc.E14-07-1238

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2015 Takada et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

“ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

PMC Copyright notice

FIGURE 5: — Localization of EGFP-Mnk and Mnk variants with DNA damage. Bleomycin and rhodamine-tubulin were injected into transgenic embryos expressing each EGFP-Mnk variant in mnk mutant background. EGFP is shown in green and rhodamine is shown in magenta in merged images. (A) EGFP-Mnk restored centrosome inactivation and mitotic delay in mnk mutant. Anastral spindles formed (time 2:30–9:20). EGFP-Mnk localized to centrosomes and formed foci/aggregates on mitotic chromosomes (time 2:30–16:30). Frames were selected from Supplemental Movie S5. (B) EGFP-D303A did not restore centrosome inactivation and mitotic delay in mnk mutant. Normal spindles formed (time 2:00–3:10). Mitotic delay did not occur; however, chromosome segregation failed, and connected dumbbell-shaped nuclei formed. The midbody did not form (time 10:00–14:30). EGFP-D303A localized to centrosomes and formed foci/aggregates on mitotic chromosomes (time 0:30–6:00) similarly to EGFP-Mnk. Frames were selected from Supplemental Movie S6. (C) EGFP-Δ[470-476] did not restore centrosome inactivation and mitotic delay in this mnk mutant embryo. EGFP-Δ[470-476] was not actively imported into the nucleus. It localized to centrosomes and formed foci/aggregates on mitotic chromosomes (time 0:50–4:40) similarly to EGFP-Mnk or EGFP-D303A. Frames were selected from Supplemental Movie S16. (D) EGFP-Δ[26-46] did not restore centrosome inactivation and mitotic delay in mnk mutant. EGFP-Δ[26-46] localized to the nucleus and centrosomes. It was excluded from the vicinity of mitotic chromosomes (time 0:40–4:00) as shown without DNA damage (Figure 4E). It did not make foci/aggregates on mitotic chromosomes. Frames were selected from Supplemental Movie S14. Elapsed time is shown in minutes:seconds. NEB is set as time 0:00. Scale bar, 10 μm.