TABLE 3:

Summary table.

	FH2 location	Fertility rate (% hatched)	Stable dimer?	Polymerization rate at t1/2 (a.u./s)	Elongation rate (subunits/s)	koff × 10−5 (s−1)	Characteristic run length (μm)a	Sequence conservationb
WT	N/A	88	+	65.8	27.0 ± 3.2	24.5 ± 0.4	300 ± 40	N/A
K586M	Lasso	25	+	54.4	19.3 ± 1.5	85.2 ± 2.3	62 ± 5	++
D854N	Post	19	+	22.2	25.5 ± 4.1	59.2 ± 2.0	120 ± 20	++
P597L	Lasso	18	+	27.9	19.7 ± 3.2	210.0 ± 6.2	26 ± 4	c
H977Y	Post	9	+/-	46.7	19.1 ± 2.8	130.0 ± 2.3	40 ± 6	–
D662N	Lasso	2	+	65.9	24.3 ± 2.7	29.7 ± 0.9	220 ± 30	+
L768H	Knob	1	–	n.d.	n.d.	n.d.	n.d.	++
I751T	Knob	0	–	16.6	25.3 ± 2.9	277.0 ± 4.8	25 ± 3	++
Δloop	Lasso	n.d.	+	90.2	30.9 ± 3.7	≥ w.t.	≥ w.t.	–

n.d., not determined.

^aCharacteristic run length = elongation rate (subunits s⁻¹)/k_off (s⁻¹)/365 subunits μm⁻¹.

^b Degree of sequence conservation was determined by analyzing our sequence alignment (Supplemental Figure S1) with ESPript (http://espript.ibcp.fr; Robert and Gouet, 2014). ++, residues with similarity >70%; +, residues with similarity >30% but <70%; –, no apparent conservation.

^cPro-597 is only conserved within the FMN family.