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. 2015 May 15;26(10):1901–1917. doi: 10.1091/mbc.E14-09-1384

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© 2015 Groen et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 2: — GFP-Fascin is dynamic in the nucleus during S10B. (A, C) Representative examples of FRAP experiments. Single-slice confocal images of GFP-Fascin (UAS GFP Fascin/oskarGal4) during FRAP time course showing prebleach, postbleach, half of fluorescence intensity recovery, and recovery endpoint. (B, D) Representative FRAP recovery curves plotting relative fluorescence intensity over time. (A, B) Bleach of whole nucleus. FRAP reveals that Fascin is transported into the nucleus during S10B, as fluorescence signal recovers after ∼8 min when a whole nucleus is bleached. (C, D) Bleach of small nuclear region (yellow box). Fascin is also dynamic in the nucleus, as a small bleached region recovers fluorescence intensity very rapidly.