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. 2015 May 15;26(10):1947–1956. doi: 10.1091/mbc.E14-11-1497

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© 2015 ErLin et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 1: — Msi-1 is specifically expressed in Sertoli cells. (A) In mice, Msi-1 was predominantly expressed in the brain and testes, with lower levels detected in the spleen and pancreas. (B) Immunoblotting of Msi-1 in lysates from adult mouse testes, Sertoli cells, germ cells, and Thy1⁺ spermatogonia; β-tubulin served as a loading control. (C) Immunohistochemical staining of Msi-1 in both immature testes (b) and the seminiferous epithelium of adult mouse testes (c); (a) negative control. The # in b indicates germ cells; * in c indicates Sertoli cell nuclei. spg, spermatogonia; PD, postnatal day. Scale bars: 25 μm.