FIGURE 4:
Msi-1 is accumulated in stress granules. (A–D) In the untreated Sertoli cells (a–c), Msi-1 (red) and stress granule markers (green), including TIAR (A), eIF3 (B), eIF4G (C), and HuR (D) were detected throughout the cytoplasm of Sertoli cells. In heat-treated Sertoli cells (a′–c′), these stress granule markers were assembled into Msi-1–positive cytoplasmic granules (white arrows). The nuclei were visualized with DAPI (blue). Scale bars: 10 μm.