Fig. 4.

Dissociated ventromedial hypothalamic nucleus (VMN) neurons from P24 DIO and DR rats fed chow or HE diet (n = 8–10 rats/group) for 3 days were categorized using calcium imaging as being oleic acid (OA)-excited (OAE, A, B) or OA-inhibited (OAI, C, D) using 15 nmol/l OA at 2.5 mmol/l glucose. They were then categorized as being β-OHB excited (β-OHBE) (A, C) or β-OHB inhibited (β-OHBI) (B, D), and their sensitivity to β-OHB was assessed by the alteration in intracellular Ca²⁺ concentration ([Ca²⁺]_i) oscillation produced by successive exposure to 0.1 nmol/l, 1 nmol/l, 10 nmol/l, 100 nmol/l, and 1 μmol/l OA. Data are expressed as percent neuron ± SE tested in each category. ^a,bData points with differing superscripts at each β-OHB concentration differ from each other by P <0.05 or less by Bonferroni post hoc test after intergroup differences were found after two-way ANOVA.