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gCTRP9 is the active form of CTRP9 protein. A: treatment with gCTRP9 (0.5 μg/ml) but not fCTRP9 (3 μg/ml) for 30 min activates AMPK, Akt, and eNOS in adult cardiomyocytes. B: treatment with fCTRP9 (3 μg/ml) for 2 h activates AMPK, Akt, and eNOS. Cotreatment with protease inhibitor (PI) cocktail blocked kinase activation by fCTRP9; n = 10–12 dishes from ≥3 mice/group. **P < 0.01 vs. control; ##P < 0.01 vs. fCTRP9.
