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. 2015 Mar 11;308(10):F1119–F1127. doi: 10.1152/ajprenal.00543.2014

Fig. 3.

Fig. 3.

Effect of aldosterone (Aldo) infusion on total NCC, phospho-NCC, phosphorylations of ERK1/2 and OSR1 in SPAK KO mice. SPAK KO mice were fed with NS diets and aldosterone osmotic minipumps (250 μg·kg−1·day−1) were implanted for total 14 days before the kidney tissues were harvested. A: representative immunoblot for total NCC, phospho-ERK1/2, total ERK1/2, and dual-specificity protein phosphatase 6 (DUSP6) in sham surgery or aldosterone minipump group. B: bar graph representation of average band density from A. **P < 0.01; n = 7. C: representative immunoblot for total NCC (t-NCC), phospho-NCC (pT53-NCC), total OSR1 and phospho-OSR1 (pS325OSR1). D: bar graph representation of average band density from C. *P < 0.05; n = 7.