Fig. 5.

Effect of knocking down DUSP expression on total NCC and ERK1/2 phosphorylation in mDCT cells. The mDCT cells were transfected with siRNA DUSP6 (10 nM). Forty-eight hours after transfection cells were lyzed for Western blot analysis. A: representative immunoblot for expressions of total NCC, phospho-ERK1/2, total ERK1/2, and DUSP6 in mDCT cells in both siRNA DUSP6 and scramble control groups. B: bar graph representing average band density from 4 independent experiments. *P < 0.05, **P < 0.01 compared with control group; n = 6.