Fig. 5.

HG effect on DNA-binding activity of HNF4α in human MCs (electrophoretic mobility shift assay). A: representative experiment. Nuclear extracts were extracted from MCs treated with NG or HG for 6 days. Unlabeled oligonucleotides were 50-fold greater than labeled oligonucleotides. B: summary data from five independent experiments (n = 5) showing HNF4α expression levels in cells treated with NG and HG plus labeled probes (lanes 2 and 5 in A). The HNF4α expression level in HG + labeled probe-treated MCs was normalized to that in NG + labeled probe-treated MCs. *P < 0.05 vs. NG + labeled probe.