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. 2015 Mar 18;308(10):F1135–F1145. doi: 10.1152/ajprenal.00563.2014

Fig. 8.

Fig. 8.

Effect of knocking down HNF-4α on fibronectin (FN) and collagen type IV (Col IV) protein expression in human MCs. A: representative Western blots showing FN expression in MCs without transfection or transfected with siRNA against human HNF4α (siHNF4α) or scrambled siRNA (Scr). Actin was used as a loading control. B: summary data from the experiments shown in A. FN expression levels were normalized to actin. *P < 0.05 compared with both Untran and Scr groups. n is the number of independent experiments. C: representative Western blots (duplicate) showing Col IV expression in MCs without transfection or transfected with siRNA against human HNF4α (siHNF4α) or scrambled siRNA (Scr). Actin was used as a loading control. D: summary data from the experiments shown in C. Col IV expression levels were normalized to actin. *P < 0.05 compared with both Untran and Scr groups. n is the number of independent experiments.