Table 1.
Gene | Forward (5′-3′) | Reverse (5′-3′) |
---|---|---|
CAV1 | CGCACACCAAGGAGATTGA | CCATAGGGATGCCGAAGATG |
CCR2 | GTTCTCTTCCTGACCACCTTC | GGGCATTGTTTGCAGAGATTT |
HPRT1 | TAGGTCCATTCCTATGACTGTAGA | TGGCCTGTATCCAACACTTC |
IL-1β | ATCTATACCTGTCCTGTGTGATG | GACAGGTCTGTGCTCTGC |
IL-10 | CGACGCTGTCATCGATTTCT | AGCTCTATTTATGTCCTGCAGTC |
MCP1 | GTCTCAGCCAGATGCAGTTAAT | GCTGAAGTCCTTAGGGTTGATG |
Muc2 | GGAACTTCAGAAAGAAGCCAGAT | CGGACTCTATGCTGGAGTGA |
RPS18 | CGAGAAGTTTCAGCACATCCT | GAACCAGTCAGGGATCTTGTATT |
TNF-α | ACCTTATCTACTCCCAGGTTCTC | CGGACTCCGTGATGTCTAAGTA |
ZO1 | GCAATTCCGTATCGTTGGATTT | CGGTTGTATGCTGGAGTGAT |
Real-time PCR primers were designed using Integrated DNA Technologies Primer Quest and Oligo Analyzer tools.