(A) Representative image showing axons (green) and blood vessels (red) after
hemisection SCI. The yellow square denotes: the monitored microvessel at the
edge of lesion site. (B) In vivo two-photon imaging of a microvessel
proximal to the lesion site at 30 min, 60 min, 90 min and 120 min
post-injury. (C) In vivo two-photon imaging of regional microvascular blood
flow from the sham group, saline-treated SCI group and the MP-treated SCI
group at 30 min, 60 min, 90 min and 120 min post-injury. (D) Representative
images depict blood vessels (red) at the edge of lesion sites in
saline-treated group and MP-treated group. Nuclei were stained with DAPI
(blue). Asterisk indicates lesion site. (E) Changes of vascular lumen
diameter in the sham group (n = 6), saline-treated group (n = 6), and
MP-treated group (n = 6) at 30 min, 60 min, 90 min and 120 min post-injury.
P > 0.05. (F) Changes of microvascular blood flow velocity in the
sham group (n = 6), saline-treated group (n = 6), and MP-treated group (n =
6) at 30 min, 60 min, 90 min and 120 min post-injury. Values presented are
mean ± SEM. *P < 0.01. Repeated measure ANOVA followed
by Fisher's LSD. (G) The number of microvessels at the edge of
lesion site in saline-treated (n = 6) and MP-treated (n = 6) mice. Values
presented are mean ± SEM. *P < 0.01.
Student's t test.