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. 2015 May 19;5:9691. doi: 10.1038/srep09691

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(A) In vivo two-photon imaging of calcium influx in the sham group and injured axons in the saline-treated SCI group and the MP-treated SCI group at 30 min, 60 min, 90 min and 120 min post-injury. (B) Western blots of calpain-1 and cleaved caspase-3 expression in injured spinal cord segment of saline-treated group and MP-treated group. (C) Changes of calcium influx after hemisection SCI in sham group (n = 6), saline-treated (n = 6) and MP-treated (n = 6) mice at 30 min, 60 min, 90 min and 120 min post-injury. Values presented are mean ± SEM (P < 0.05). Statistical analysis: repeated measure ANOVA. Fifteen to twenty axons were measured individually per animal. (D) Quantification of protein band density to determine levels of active calpain-1 and cleaved caspase-3. *P < 0.01. Student's t test.