Table 3.
Comparison of the 454 tagged pyrosequencing of the pooled samples from the same species and the same season, generated through either pre-digestion of the DNA using restriction enzymes (PvuII and MscI) or oligonucleotide blockers designed to inhibit the amplification of plastid and mitochondrial 16S rRNA.
| Species | Season | Digest | Digest | Blocker | Blocker | Phyla |
|---|---|---|---|---|---|---|
| Reads | %Org | Reads | %Org | Correlation | ||
| Acer negundo | W | 32260 | 86.75 | 24090 | 67.25 | 0.99 |
| S | 20959 | 2.05 | 19744 | 2.3 | 1.00 | |
| F | 11789 | 82.35 | 16821 | 68.3 | 0.95 | |
| Ulmus parvifolia | W | 4520 | 17.9 | 14121 | 13.35 | 0.97 |
| S | 4752 | 22.05 | 12535 | 22.15 | 0.44 | |
| F | 11 | − | 21735 | 79.25 | NA | |
| Ulmus pumila | W | 4520 | 32.7 | 19486 | 64.4 | 0.07 |
| S | 3987 | 36.35 | 14330 | 14.8 | 0.96 | |
| F | 2299 | 49.45 | 22677 | 83.65 | 0.88 |
The OTUs from both sample sets were rarified to 2000 when estimating organelle read content.