Table 2.
MicroCT analysis of femora trabecular and cortical bone parameters.
| Mice group | Sham-Ve (n = 12) | Sham OT 1 mg/kg (n = 12) | ORX Ve (n = 12) | ORX OT 1 mg/kg (n = 12) | |
|---|---|---|---|---|---|
| Trabecular bone | |||||
| Distal femoral metaphysis | BV/TV (%) | 15.4 ± 1.2 | 11.9 ± 1.2 | 3.2 ± 0.3a | 3.1 ± 0.3a |
| Tb. Th. (μm) | 69 ± 1 | 63 ± 2a | 65 ± 1a | 62 ± 1a | |
| Tb. N. (mm−1) | 2.2 ± 0.1 | 1.9 ± 0.1 | 0.5 ± 0.04a | 0.5 ± 0.04a | |
| Tb. Sp. (μm) | 238 ± 9 | 247 ± 6 | 532 ± 23a | 528 ± 16a | |
| Cortical bone | |||||
| Mid-femur diaphysis | CSA (mm2) | 1.04 ± 0.08 | 0.97 ± 0.06a | 0.83 ± 0.04a | 0.83 ± 0.04a |
Control (Sham) and ORX mice were submitted to daily injections of OT or Ve for 8 weeks starting 2 weeks after surgery. Detailed analysis of trabecular parameters was performed on the distal metaphysis of femora and on the mid-femur diaphysis for cortical bone parameters. Bone volume/total volume (BV/TV), trabecular thickness (Tb. Th), trabecular spacing (Tb. Sp), trabecular number (Tb. N), and cortical cross-sectional area (CSA) are reported in the Table 1.
ap < 0.05 vs. Sham-Ve (n = 12 mice/group). Data are represented as mean ± SEM.