Skip to main content
. Author manuscript; available in PMC: 2015 May 19.
Published in final edited form as: Methods Mol Biol. 2011;776:313–331. doi: 10.1007/978-1-61779-243-4_18

Figure 4.

Figure 4

Image segmentation using Pipeline Pilot. A) Raw images are grouped into an image reader. B) Nuclear and C) whole cell masks are created to extract cell-by-cell measurements D) with removal of edge touching regions. E) Cytoplasmic masks are generated by subtraction the nuclear mask from the whole cell masks. Filtering (gray strikethroughs) is used to discard dead cells or mis-segmented objects.