Figure 1. Characterization of TCD1.

(a) TCD1-Derived Protein Sequence. CD1 (chromodomain 1) and CD2 (chromodomain 2) are the regions that are similar to the canonical chromodomain. Both sequences are underlined. CSD is the chromoshadow domain. (b) Expression pattern of the TCD1 gene. Total RNA from cells undergoing vegetative growth (G), starvation (S), and conjugation (at 0 h, 2 h, 4 h, 6 h, 8 h, 10 h, 12 h, 14 h, 16 h, and 24 h) after mixing were used. The obtained fragments were amplified by RT-PCR with primers for the TCD1 coding sequences. (c) Northern blot probed with the TCD1 coding sequence. The lower panel shows the loading control of rRNA stained with ethidium bromide before blotting. The samples derive from the same experiment and that gels have been run under the same experimental conditions and were processed in parallel.