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. 2015 May 19;5:10243. doi: 10.1038/srep10243

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Copyright © 2015, Macmillan Publishers Limited

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HA-TCD1-B cells and WT cells (CU428) were mated. Cells collected at 3 h, 5 h, 7 h, 9 h, 12 h, and 14 h after mixing were fixed and processed for immunofluorescence staining with anti-HA primary and FITC-conjugated secondary antibodies (middle column). Cells were also stained with DAPI to visualize DNA (left column). Cells underwent pair formation (panel 1), crescent (panel 2), meiosis I (panel 3), meiosis II (panel 4), anlagen (panel 5, 6, 7), pair separation (panel 8). Scale bar, 10 μm.