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. 2015 May 19;5:10243. doi: 10.1038/srep10243

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HA-Tcd1 cells and WT (CU428) were mated and collected at 10 h (1), 12 h (2) and 14 h (3) after mixing. Mating cells were fixed and processed for immunofluorescence with rabbit anti-K9 trimethyl-histone H3 polyclonal antibody and FITC-conjugated secondary antibody (green), or mouse anti-HA monoclonal antibody and Alexa-conjugated secondary antibody (red). Scale bar, 10 μm.