Figure 5.

Electropherograms of PCR products using the TP-PCR forward and reverse primer combinations illustrating sizing capabilities. (a) and (b) show electropherograms derived from using the TP-PCR forward and reverse primer combinations, respectively. (a) shows an enlarged electropherogram image of sample 58 with repeat lengths 5 and 140; illustrating the ability to visualise the expanded allele peak, within the clinically mild range, at the right-hand end of the characteristic ladder profile. (b) shows two clear allele peaks illustrating the ability to size alleles within the unaffected range with the TP-PCR reverse primer combination.