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. Author manuscript; available in PMC: 2015 Jul 15.
Published in final edited form as: Cancer Res. 2014 May 15;74(14):3695–3706. doi: 10.1158/0008-5472.CAN-13-2591

Fig. 4. Slug inhibition or knockdown leads to apoptotic cell death.

Fig. 4

(A) Control or Slug-sh1 expressing PyMT-N-cad metastatic cells were treated with DMSO or PD173074 (FGFRi) at the indicated concentrations. Cell lysates were immunoblotted for the indicated proteins. (B) PyMT-N-cad cells expressing Slug-shRNA were further transduced with either empty vector (pLXSN) or mouse Slug (pLXSN-Slug) to rescue Slug expression. Cells were then treated with PD173074 and immunoblotted as in A. (C) Control and Slug-sh1 PyMT-N-cad cells were treated with DMSO or 1 μM doxorubicin for 16 hrs. Fixed cells were stained for active-caspase-3 and DAPI. (D) The number of caspase-3 positive cells is shown as Mean ± SEM (N=3). Unpaired t-test, ***P=0.0003. (E) Control or Slug-sh1 PyMT-N-cad cells were treated with DMSO, 0.5 μM doxorubicin or PD173074 (FGFRi), or both for 18 h. The levels of active-caspase-3 or cleaved-PARP were analyzed by immunoblotting.