Table 2.
Additional proteins identified in all data sets with a predicted role in carbohydrate uptake
| Accession number |
Protein name | Locus tag | MW (kDa) |
PSORTb 3.0 |
H7 NSAF % |
APM NSAF % |
GLU NSAF % |
Fold- change H7/GLU |
Fold- change APM/GLU |
H7:GLU t-test p-value |
APM:GLU t-test p-value |
|---|---|---|---|---|---|---|---|---|---|---|---|
| D6E1S8 | Glucose/Galactose ABC transporter substrate-binding protein | EUR_01240 | 46 | U | 1.156 ± 0.04 | 0.763 ± 0.042 | 0.522 ± 0.048 | 2.21 | 1.46 | 0.000 | 0.003 |
| D6E1S9 | Monosaccharide ABC transporter ATP-binding protein | EUR_01250 | 56 | CM | 0.691 ± 0.061 | 0.355 ± 0.042 | 0.471 ± 0.065 | 1.47 | 0.75 | 0.013 | 0.061 |
| D6E1T0 | Monosaccharide ABC transporter membrane protein | EUR_01260 | 60 | CM | 0.754 ± 0.092 | 0.490 ± 0.015 | 0.285 ± 0.083 | 2.65 | 1.72 | 0.003 | 0.013 |
| D6E3P2 | PTS system IIA component, Glc family | EUR_08690 | 17 | CM | 0.134 ± 0.012 | 0.104 ± 0.023 | 0.120 ± 0.027 | 1.12 | 0.87 | 0.466 | 0.488 |
| D6E3P3 | PTS system N- acetylglucosamine- specific IIB component, Glc/ maltose family | EUR_08700 | 52 | CM | 0.762 ± 0.055 | 0.592 ± 0.051 | 0.306 ± 0.056 | 2.49 | 1.94 | 0.001 | 0.003 |
| D6E440 | PTS system IIA component, Glc family | EUR_10290 | 80 | CM | 0.219 ± 0.01 | 0.149 ± 0.011 | 0.304 ± 0.031 | 0.72 | 0.49 | 0.011 | 0.001 |
| D6E521 | Carbohydrate ABC transporter substrate-binding protein | EUR_14180 | 46 | U | 0.081 ± 0.01 | 0.067 ± 0.009 | 0.113 ± 0.042 | 0.72 | 0.59 | 0.271 | 0.138 |
| D6E581 | ABC transporter substrate-binding protein (MalE) | EUR_14810 | 57 | U | 0.154 ± 0.024 | 0.077 ± 0.017 | 0.067 ± 0.020 | 2.32 | 1.16 | 0.008 | 0.519 |
| D6DYZ3 | PTS system D- fructose-specific IIA component | EUR_28570 | 67 | CM | 0.125 ± 00.026 | 0.105 ± 0.003 | 0.133 ± 0.022 | 0.94 | 0.80 | 0.720 | 0.107 |
| D6DZQ3 | Carbohydrate ABC transporter substrate-binding protein | EUR_31580 | 64 | U | 0.117 ± 0.022 | 0.103 ± 0.025 | 0.179 ± 0.023 | 0.65 | 0.58 | 0.027 | 0.017 |
| D6DZZ0 | PTS system, lactose/cellobiose family IIC component | EUR_32520 | 47 | CM | 0.010 ± 0.017 | 0.000 ± 0.00 | 0.000 ± 0.00 | H7 | H7 | 0.374 | N/A |
Only proteins of COG G (Carbohydrate transport and metabolism) displayed. Fold change was calculated based on the mean NSAF from three replicates. Only proteins that had 5 or more total spectra in at least one set of samples (H7 or APM) were included. PSORTb 3.0 was used to predict cellular location: U=Unknown, CM=Cell Membrane, C=Cytoplasmic, CW=Cell Wall. NA = not applicable. Rows in bold print denote proteins that were selected for further study.